DNA Extraction – The Start of Molecular Identification

Earlier, we shared that we had successfully isolated pure strains of microalgae as part of our project. We also mentioned that we began accumulating biomass for molecular identification (DNA barcoding).

📦 Now it’s time for the next step! We’ve started DNA extraction from the collected biomass, which involves several sequential steps:

1️⃣ Cell wall and membrane lysis, followed by precipitation and removal of cell debris
2️⃣ Binding of DNA to a membrane in a spin column
3️⃣ Washing, drying, and elution of DNA

This stage requires a lot of lab equipment: ultrasonic disruptor, mill, flow box and fume hood, many micro-pipettes, different stands and microtubes, a few centrifuges and vortex mixers, a block heater, and a thermo-shaker. But the most important things are: solid methodological knowledge, an individual approach to each microalgal strain — and skillful hands.

⚗️ After that comes quality control: we will assess the concentration and purity of the DNA using spectrophotometry and gel electrophoresis.

🧬 The next steps are PCR amplification, purification, and sending the samples for sequencing. Then begins the world of bioinformatics — where the genetic data will be analyzed.

📡 Follow our updates — the most exciting parts are still ahead!

#QuarryLifeAward #QLA6